Agarose gel electrophoresis.
نویسنده
چکیده
Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.
منابع مشابه
Agarose Gel Electrophoresis for the Separation of DNA Fragments
Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose) subunits(2). During gelation, agarose polymers associate non-covalently and form a network of bundles whose pore sizes determine a gel's mo...
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This section describes the application of agarose gel electrophoresis to both analytical and preparative separation of DNA fragments. Standard agarose gels separate DNA fragments from ∼0.5 to 25 kb, whereas pulsed-field agarose gels resolve molecules from ∼10 to >2000 kb. Descriptions of standard and pulsed-field agarose gel electrophoresis as well as parameters affecting resolution of large DN...
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Microsatellite markers are employed for genotyping of Basmati varieties and assaying purity of market samples. However, employment of diverse electrophoresis techniques across laboratories has resulted in inconsistent allele sizes, creating doubts about the suitability of the assay. This study evaluated agarose gel electrophoresis, slab gel electrophoresis, and capillary electrophoresis techniq...
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Preparative gel electrophoresis of double-stranded DNA usually includes staining the gel with ethidium bromide followed by illumination with ultraviolet (UV-B) light. In this report, DNA isolated from agarose gels was found to be a poor substrate for in vitro transcription, transformation of E. coli and PCR. Inhibition was not caused by enzyme-inhibiting impurities in the agarose gel, but was i...
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In this work, a novel agarose gel electrophoresis strategy has been developed for separation of DNA fragments by doping graphene oxide (GO) into agarose gel. The results show that the addition of GO into agarose gel significantly improved the separation resolution of DNA fragments by increasing the shift distances of both the single DNA fragments and the adjacent DNA fragments and completely el...
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ورودعنوان ژورنال:
- Methods in enzymology
دوره 529 شماره
صفحات -
تاریخ انتشار 2013